Azco provides columns and bulk reagents for the analysis and purification of synthetic oligonucleotides to support you from the bench all the way to full scale GMP manufacturing.

Oligonucleotide Analysis and Purification
 

Depending on the synthetic methodology used, oligonucleotides can be purified many ways including:

·         Reverse Phase LC

·         Ion-exchange LC

·         Gel-filtration Chromatography

·         Ultra-filtration 

Azco supplies AMBERCHROM™ and AMBERLITE™ brand polymeric reversed phase and ion exchange resins from Rohm and Haas and also supplies OLIGOSEP™ and OLIGOPREP™ brand polymeric reversed phase columns from Transgenomic.  All of these materials have been optimized for the analysis and purification of oligonucleotides and are readily scalable to meet any peptide requirement from the bench to large scale manufacturing.  Rohm and Haas purification products are manufactured under GMP conditions with all necessary supporting regulatory .documentation.

AMBERCHROM™ resins are highly efficient materials for reversed phase chromatography without the limitations of silica.  These resins are designed for the purification of proteins, peptides and oligonucleotides.  AMBERCHROM™, reversed phase chromatography resins, are ideal for discovery, development and full scale production of biopharmaceuticals compounds.For detailson specific materials or column (low or DAC high pressure) packing procedures, please call or email Azco.

The OLIGOSep/Prep™  Family

There are two products manufactured by Transgeomic, specifically for the "high-speed", ion-pair, revesed  separation of oligonucleotides.  They are:

·         OLIGOSep™  is a 2 micron, fully alkylated, C18 polymeric reversed phase column.  This material is designed to withstand pH in the range of 0 to 14 and heating up to 100C.  Their small particle size gives them excellent efficiency for reversed phase separation, and the pH and temperagure ranges provide dimensions for separation optimization.

·         OLIGOPrep™  columns offer the same benefits as above; however, they use 5 micron particle size resins to allow for higher flow rates; thus, faster purification of oligonucletides.

The Amberchrom™  Family

·         AMBERCHROM™ XT polishing grade resins demonstrate high efficiency with improved selectivity for difficult separations when compared to silica RPC resins. They offer high capacity and high flow rates in medium to high pressure production applications.

·         AMBERCHROM™ CG chromatography grade resins.  These materials have been commercially proven for many years,  AMBERCHROM™ CG resins are suitable for use in many peptide and oligonucleotide applications including, front end capture, purification, flow-through, and desalting depending on the pore size and particle size selected.

·         AMBERLITE™resins are low cost, low efficiency, large particle size resins designed for capture or flow-through applications or any application where a low-cost adsorbent is required.

 

Amberchrom™ XT Resins 

Reversed Phase Chromatographic Resins for Bimolecular Purification and Polishing

AMBERCHROM™ XT chromatographic media are a refinement of the existing AMBERCHROM™ CG line offering higher efficiency and higher pressure capabilities. ,Iimproved resolution is provided from the smaller particle sizes.  The XT line is still pH stable over the entire range and provides the same stability to caustic CIP procedures. Theyse materials are targeted for high efficiency oligonucleotide purification whileproviding an economical and rugged alternative to silica for reversed phase chromatographic protocols.

AMBERCHROM™ XT media have demonstrated exceptional capabilities for purifying both naked as well as highly modified oligonucleotides and RNA. They are ideally suited for therapeutic oligonucleotides such as antisense or siRNA.

Downstream purification can be optimized by taking advantage of Amberchrom’s excellent pH stability in purifying a wide range of peptides and oligonucleotides.  The resolving power of AMBERCHROM™ XT delivers high yield-purity in the separation, and their superior rigidity permits use with medium pressure equipment, up to 60 bar.

Available in two different particle size ranges (20 and 30 microns), AMBERCHROM™ XT resins are compatible with most organic solvents, dilute acids and bases.

Amberchrom™ CG Resins for High Performance Polymeric RPC Chromatography

AMBERCHROM™ resins provide the purification power for reversed phase chromatography without the limitations of silica media such as pH and temperature.  Amberchrom resins are pH stable from 0 to 14 and are compatible with high temperature applications.

AMBERCHROM™ CG media are macroporous, polymeric reversed phase liquid chromatography resins. They are designed for laboratory and process scale purifications of proteins, peptides, nucleic acids, antibiotics, and small molecular weight pharmaceuticals.

Large surface area, unique pore size and pore volume distribution make AMBERCHROM™ CG300 ideally suited for the separation of peptides. This resin has high capacity for many pharmaceutical compounds and has been commercially proven for many years. Amberchrom is an excellent technical and economical alternative to RPC silica when used in high resolution, low pressure chromatography.

AMBERCHROM™ CG resins are available in three different particle size ranges: small (S), medium (M)  and coarse(C), with,  35, 75, and 120 micron mean particle sizes respectively.  These materials are supplied ready to pack as a slurry in 20 % ethanol. AMBERCHROM™ CG resins are ideally suited for operation within the entire pH range, and can be easily cleaned in place (CIP) with 1M Sodium Hydroxide. Amberchrom is compatible with most organic solvents and dilute acids and bases.

AmberLite™  Resins as Low Cost Adsorbants

AMBERLite™ resins are low cost polymeric resins designed for capture applications or those applications that call for an inexpensive adsorbant.  Amberlite resins are also pH stable an can be easily cleaned.  They are offered in the same pore sizes as the Amberchrom CG resins, but much larger particle sizes.  These materials are ideal for fast flow capture or flow-through applications.

*pStyDVB = Polystyrene DVB

 

The Transgenomic separation/purification products offered by Azco include:

Amberchrom XT Prepacked Columns

Amberchrom XT-30 Profile Column

56-1001-01                               4.6 x 250mm Amberchrom XT 30

56-1001-02                               10 x 250mm Amberchrom XT 30

56-1001-03                               22 x 250mm Amberchrom XT 30

56-1001-04                               50 x 250mm Amberchrom XT 30

Amberchrom XT-20 Profile Column

56-1002-01                               4.6 x 250mm Amberchrom XT 20

56-1002-02                               10 x 250mm Amberchrom XT 20

56-1002-03                               22 x 250mm Amberchrom XT 20

56-1002-04                               50 x 250mm Amberchrom XT 20

Amberchrom HPR 10 Profile Column

56-1003-01                             4.6 x 250mm Amberchrom HPR 10

56-1003-02                             10 x 250mm Amberchrom HPR 10

56-1003-03                             22 x 250mm Amberchrom HPR 10

56-1003-04                             50 x 250mm Amberchrom HPR 10

Amberchrom XT                                            

51-2000-XX                            Amberchrom XT20                             

51-2002-XX                            Amberchrom XT30

Note: XT20 and XT30 materials available in prepacked columns also (see above).                      

AmberchromCG                                            

51-1006-XX                              Amberchrom CG161S                         

51-1004-XX                              Amberchrom CG161M                        

51-1002-XX                              Amberchrom CG161C   

51-1012-XX                              Amberchrom CG300S

51-1010-XX                              Amberchrom CG300M

51-1008-XX                              Amberchrom CG300C

51-1000-XX                              Amberchrom CG1000S

51-1018-XX                              Amberchrom CG71S
51-1016-XX                              Amberchrom CG71M                          

51-1014-XX                              Amberchrom CG71C                           

AmberLite

51-3000-XX                              AmberLite FPC22         cation exchange hydrogen form

51-3002-XX                              AmberLite FPA90         anion exchange chloride form

51-3004-XX                              AmberLite FPA91         anion exchange chloride form

51-3006-XX                              AmberrLite FPA51        anion exchange

51-3010-XX                              AmberLite FPA55         anion exchange

51-3024-XX                              AmberLite FPA40         anion exchange chloride form

51-3026-XX                              AmberLite FPC3500      cation exchange weak acid

51-3008-XX                              AmberLite XAD1600      reversed phase

51-3016-XX                              AmberLite XAD16         reversed phase same as CG161

51-3018-XX                              AmberLite XAD1180      reversed phase

51-3020-XX                              AmberLite XAD7 HP       reversed phase

Where

These materials are offered in pack sizes from grams to tens of kilograms to meet your production requirements.  Please contact Azco to get the pack that best meets your needs.