Azco Biotech, Inc
Solutions for Nucleic Acid Synthesis and Detection
Many auxiliary reagents are used for oligonucleotide synthesis to accomplish various reactions throughtout the synthesis cycle. At the start of the cycle, a deblock reagent is used to remove the DMT group from the 5’ hydroxyl on the oligonucleotide chain. Then an activator is used to activate the phosphoramidite being added to the chain. After the activated phosphoramidite is coupled to the chain, capping reagents are used to keep unelongated chains from interfering with subsequent additions. Finally, oxidzers and sulfurization reagents are used to make the phosphate group stable.
During the synthesis cycle, low water acetonitrile is used to flush the lines and rinse out the previous reagent.
After the synthesis of the oligonucleotide is complete TFA or AMA (1:1 mixture of Methyl Amine with Ammonium Hydroxide) is used to cleave the oligonucleotide from the support then ammonium hydroxide or AMA, a mixture of ammonium hydroxide and methyl amine, is used to remove all of the remaining protecting groups on the oligonucleotide bases.
Azco provides these reagents in both premade and ready-to-use (See Bulk Reagents) formats specific to your synthesizer as well as bulk powder so that you can prepare concentrations to meet your specific needs.
The pre-formulated solutions offered by Azco are:
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Catalog # |
Product Description |
Catalog # |
Product Description |
| 17-1112-XX | Anhydrous Acetonitrile (<30 ppm water) |
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| 17-1171-XX | DCI Activator (0.25M DCI in ACN) |
17-1124-XX |
Ethyl Thio Tetrazole Activator (ETT) (0.25M in ACN) |
| 17-1130-XX | Cap A Solution (acetic anhydride, pyridine and THF) |
17-1137-XX |
Cap B Solution (n-methylimidazole in THF) |
| 17-1151-XX | Deblock Solution (0.1M TCA in DCM) |
17-1161-XX |
DCA Deblock Solution (0.1M DCA in DCM) |
17-1142-XX |
Oxidizer Solution (0.10M Iodine in THF/Pyridine/Water) |
17-1159-XX |
Mild Oxidizer Solution (0.02M Iodine in THF/Pyridine/Water) |
Where:
XX |
Size |
45 |
200 mL |
47 |
450 mL |
51 |
950 mL |
59 |
2.0 L |
68 |
4.0 L |