Azco Biotech, Inc
Solutions for Nucleic Acid Synthesis and Detection
Reagents used for Post-synthesis Modifications
many molecular biological applications including gene amplification, cloning, genetic analysis, therapeutics and diagnostics. To accommodate the vastly diverse uses, many oligonucleotides require post synthetic modifications. Post synthesis modifications include the addition of a labeling dye, the addition of a quencher, the addition of cholesterol, biotinylation, phosphorylation, the addition of a linker or spaceror any number of others. Since molecular diagnostics has become a rapidly growing field, the most popular post synthesis modification has been the addition of a dye and a quencher. The addition of a dye and quencher allows the oligonucleotide to be used for the FRET analysis of a nucleic acid sequence in real-time PCR.
Azco offers a broad array of products for the post-synthetic modification of an oligonucleotide. Some are even offered as a phosphoramidite to be incorporated directly into the oligonucleotide during the synthesis process (see under the Specialty Phosphoramidite section).
Fluorophores and Other Modifying Materials or Reagents
Catalog # Product Description Catalog # Product Description 65-0022-XX (6) FAM 65-0015-XX 5-FAM 65-0018-XX 5-FAM, SE 65-0021-XX 6-FAM, SE 65-0019-XX 5-FAM-X, SE 65-0017-XX 6-FAM-X, SE 65-0033-XX 6-JOE, SE 65-0114-XX 6-HEX 65-0115-XX 6-HEX, SE 65-0116-XX 6-TET, acid 65-0117-XX 6-TET, SE 65-0009-XX 5,6 CarboxyRhodamine 6G (CR 6G) 65-0005-XX 5-CR 6G 65-0007-XX 6-CR 6G 65-0010-XX 5(6)-CR 6G, SE 65-0006-XX 5-CR 6G, SE 65-0008-XX 6-CR 6G, SE 65-0041-XX 5(6)-ROX 65-0037-XX 5-ROX 65-0039-XX 6-ROX 65-0042-XX 5(6)-ROX, SE 65-0038-XX 5-ROX, SE 65-0040-XX 6-ROX, SE 65-0049-XX 5(6) TAMRA 65-0045-XX 5-TAMRA 65-0047-XX 6-TAMRA 65-0050-XX 5(6)-TAMRA, SE 65-0046-XX 5-TAMRA, SE 65-0048-XX 6-TAMRA, SE 65-0051-XX 5(6)-TAMRA-X, SE 65-0052-XX 65-0065-XX AzcoFluor 488, acid 65-0066-XX AzcoFluor 488, SE 65-0067-XX AzcoFluor 488, amine 65-0068-XX AzcoFluor 488, hydrazide 65-0069-XX AzcoFluor 488, C2 maleimide 65-0012-XX Dansyl Chloride 65-0070-XX AzcoFluor 555, acid 65-0071-XX AzcoFluor 555, SE 65-0072-XX AzcoFluor 555, amine 65-0073-XX AzcoFluor 555, hydrazide 65-0074-XX AzcoFluor 555, C2 maleimide 65-0075-XX AzcoFluor 647, SE 65-0076-XX AzcoFluor 647, acid 65-0077-XX AzcoFluor 647, hydrazide 65-0078-XX AzcoFluor 647, amine 65-0079-XX AzcoFluor 647, C2 maleimide 65-0080-XX AzcoFluor 680, acid 65-0081-XX AzcoFluor 680, SE 65-0082-XX AzcoFluor 680, amine 65-0083-XX AzcoFluor 680, hydrazide 65-0084-XX AzcoFluor 680, C2 maleimide 65-0085-XX AzcoFluor 750, acid 65-0086-XX AzcoFluor 750, SE 65-0087-XX AzcoFluor 750, amine 65-0088-XX AzcoFluor 750, hydrazide 65-0089-XX AzcoFluor 750, C2 maleimide 65-0090-XX Texas Red® C2 maleimide 65-0091-XX Texas Red® hydrazide 65-0092-XX DABCYL 65-0093-XX DABCYL, SE 65-0094-XX QXL 610, acid 65-0095-XX QXL 610, acid SE 65-0096-XX QXL 610, vinyl sulfone (thio reactive) 65-0097-XX QXL 610, amine 65-0098-XX QXL 490, acid 65-0099-XX QXL 490, acid SE 65-0100-XX QXL 490, C2 maleimide 65-0101-XX QXL 490, C2 amine 65-0102-XX QXL 570, acid 65-0103-XX QXL 570, acid SE 65-0104-XX QXL 570, C2 maleimide 65-0105-XX QXL 570, C2 amine 65-0106-XX QXL 670, acid 65-0107-XX QXL 670, acid SE 65-0108-XX QXL 670, C2 amine 65-0109-XX